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. 2022 Mar 2;34(6):2188–2204. doi: 10.1093/plcell/koac078

Figure 9.

Figure 9

Hysteresis of ELF3 drives hysteresis in PIF4 promoter activity. A–C, Response of the luminescence driven by pPIF4:LUC (A and C) or pHY5:LUC (B) in the Col-0 (A and B) or elf3 (C) backgrounds to increasing or decreasing temperature. D and E, Response of the number of ELF3 nuclear speckles (square root-transformed data) from hypocotyl cells of seedlings expressing p35S:YFP-ELF3 to increasing or decreasing temperature. See Supplemental Figure S5 for information on the kinetics of ELF3 speckles and their response to nighttime temperature during the morning. F, Correlation between luminescence driven by pPIF4:LUC (from A) and the number of speckles driven by p35S:YFP-ELF3 (from D). The seedlings were exposed to white light at either 10°C or 28°C and 4 h after the beginning of the photoperiod were transferred to the temperature indicated on the x-axes (including controls that remained at 10°C or 28°C). Luminescence (A–C) or confocal images (D and E) were taken 3 h later. Data are means ± se of three plates with seedlings (A–C) or 15 replicate boxes with seedlings (D and E). E, Representative confocal images. Scale bar = 2.61 µm. In (F), the correlation is significant at P = 0.0002. See Supplemental File S1, T–X for detailed statistics.