Figure 1.

Study Protocol. Colored boxes represent scheduled sleep and underlines represent scheduled wakefulness. The black box on study day 14 indicates a recovery sleep opportunity required for participants in condition order A before discharge from the lab. Blood draws for metabolomics analyses are represented by T1, T5, T9, T13, T17, and T21, where the number refers to hours since scheduled waketime (e.g. T5 = 5 hours since scheduled waketime). All protocol events were scheduled according to each participant’s habitual bedtime. For example, the T1 blood collection for a subject would occur at 10:00am if their wake time was scheduled for 9:00am, while another subject’s T1 blood collection would occur at 8:00am if their wake time was scheduled for 7:00am.