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. 2022 May 12;94(20):7339–7349. doi: 10.1021/acs.analchem.2c00765

Figure 3.

Figure 3

Reproducibility of partial RNase T1 digests of mRNA. (A) Total ion chromatograms of the partial RNase T1 digests of eGFP mRNA. Twenty micrograms of mRNA was incubated with 2.5 μL of immobilized RNase T1 for 10 min at 37 °C prior to LC–MS/MS analysis. The number of unique oligoribonucleotides and % sequence coverage are highlighted in each replicate. (B) Extracted ion chromatogram of three identified unique oligoribonucleotides from the LC–MS/MS analysis. The retention time and RSD across the replicates are shown for each oligoribonucleotide.