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. 2022 May 12;13:874878. doi: 10.3389/fimmu.2022.874878

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Copyright © 2022 Xia, Shi, Qian, Han, Lu, Tao, Gu, Zhao, Wei and Lu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FMT helped restoring intestinal barrier function and ameliorating hepatic inflammation. (A) Experiment design. (B) Immunofluorescence analysis on ZO-1, E-cadherin and Claudin-1 in colon sections from different groups. Representative images are shown. Scale bar, 100μm. (C) Relative mRNA expression of tight junctions and cell adhesion protein in colon. (D, E) ALT and AST levels in serum (ALT, n = 6. AST, n = 4, One-way ANOVA). (F) Relative mRNA expression of cytokines in liver (n = 6, One-way ANOVA). (G, H) Quantification of immune cells in the liver represented as percentages of CD45+ cells (n = 6-8). *p < 0.05, **p < 0.01, ***p < 0.001, compared with Control group. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, compared with M→C group. FMT, fecal microbiota transplantation.