FIG. 2.

(A) Organization and regulation of the alk genes on the OCT plasmid in P. oleovorans GPo1. The regulatory protein AlkS is activated by octane or DCPK and induces transcription from the alkBp promoter. The directions of transcription are indicated by arrows with open arrowheads. Transcriptional regulation of alkT has not been described. (B) Alkane degradation by enzymes encoded by alk genes. Alkanes are converted to alkanols, alkanals, and the corresponding carboxylic acids, which are coupled to coenzyme A (CoA). R represents pentyl to undecyl residues. The functions of AlkF and AlkL are unclear. (C) Detailed structure of pBG11. The region sequenced is indicated by the solid line, while the previously described sequence is indicated by the dashed line. The coordinates (in the deposited sequence) are indicated at the top together with the direction of transcription of alkS. The solid bar indicates the portion of the plasmid that was derived from pJRD158. Restriction sites that were used for cloning in this work and sites which also occur in the pUC18 polylinker are indicated at the bottom. The EcoRI, XhoI, and ApaI sites were derived from the polylinker of pGEM-7Zf(+), which was the basis of pBG11. Two modifications introduced by site-directed mutagenesis are indicated at the bottom; the sequences of newly introduced (in parentheses) or removed restriction sites are underlined. Abbreviations: Ap, ApaI; E, EcoRI; Nd, NdeI; Pa, PacI; Ps, PstI; Pv, PvuII, Sc, SacI; Sp, SphI; Xb, XbaI; Xh, XhoI.