TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| E. coli strains | ||
| X7026 | F− Δ(gpt-lac)5 supE44 relA1 spoT1 | 23 |
| JM101 | supE thi-1 Δ(lac-proAB) F′[traD36 proAB+lacIqlacZΔM15] | 44 |
| CJ236 | dut-1 ung-1 thi-1 relA1/pCJ105(Cmr F′) | 29 |
| Plasmids | ||
| pUC18 | lacZα, cloning vehicle, Apr | 57 |
| pUC18Not | lacZα, cloning vector, polylinker flanked by NotI sites, Apr | 22 |
| pUC18Sfi | lacZα, cloning vector, polylinker flanked by SfiI sites, Apr | 22 |
| pHP45Ω | Source of the T4 transcriptional terminator, Smr Apr | 39 |
| pGEM-7Zf(+) | lacZα, cloning vector, phage f1ori, Apr | Promega |
| pGEM-7Zf(−) | Same as pGEM-7Zf(+) but f1 ori is inverted, Apr | Promega |
| mini-Tn5Km | Source of kanamycin interposon, Apr Kmr | 8 |
| pBR322 | Cloning vehicle, Apr Tcr | 3 |
| pGC2 | pBR322 derivative with M13ori, Apr | 34 |
| pMC1871 | pBR322 derivative with promoterless lacZ, Tcr | 6 |
| pCKO4 | Source of xylN, Cmr | 37 |
| pBG63 | Source of xylMA, f1ori, Apr | 55 |
| pGEc258 | pBR322/pJRD158 derivative, source of alkS, Apr | 11 |
| pGEc286 | Promoter probe vector with alkBp-CAT fusion, Apr | 25 |
| pGC2Mlu | pGC2 with additional MluI site, Apr | This study |
| pBG11 | pGEM7-Zf(+) with alkS, Apr | This study |
| pBG11lacZ | pBG11 derivative with alkS′-′lacZ fusion, Apr | This study |
| pBG4 | pUC18 with T4 transcriptional terminator of pHP45Ω, Apr | This study |
| pBG4ΔN | pBG4 without NdeI site, Apr | This study |
| pBG4NΔN | pBG4ΔN with xylN of pCKO4, Apr | This study |
| pBGL | pBG4NΔN with small new polylinker, Apr | This study |
| pGEMPalkN | Cloning vector with alkBp promoter and NdeI site on the ATG of alkB, Apr | This study |
| pBGPalk | pBGL with alkBp of pGEMPalkN, Apr | This study |
| pBG11EΔN | pBG11 with an additional EcoRI site and removed NdeI site internal to alkS, Apr | This study |
| pUC18NΔN | pUC18Not without NdeI, Apr | This study |
| pUC18NS* | pUC18NΔN with alkS* of pBG11EΔN, Apr | This study |
| pSPZ1(+) | pGEM-7Zf(+) with a new polylinker, Apr | This study |
| pSPZ1(−) | Same as pSPZ1(+) but from pGEM-7Zf(−), Apr | This study |
| pSPZ2Not | pUC18NS* with alkBP and T4t of pBGPalk, contains alkS* alkBp T4t as a NotI cassette, Apr | This study |
| pSPZ2Sfi | Same as pSPZ2Nde but SfiI sites instead of NotI sites, Apr | This study |
| pBG63N | pBG63 with NdeI site in xylM removed and new NdeI site on the ATG of xylM*, Apr | This study |
| pSPZ2MA | pSPZ2 with xylM*A of pBG63N under alkBp control, Apr | This study |
| pBRNS | pBR322 with rrnBT1 terminator and additional NotI and SfiI restriction sites, Apr | This study |
| pBRNSKm | pBRNS with kanamycin resistance of mini-Tn5Km replacing the bla gene, Kmr | This study |
| pBRNSKmΔ | pBRNSKm without remnants of the tet gene, Kmr | This study |
| pSPZ3 | pBRNSKmΔ with alkS* alkBp xylM*A of pSPZ2MA, Kmr | This study |