TABLE 2.

Oligonucleotides used in this study

Oligonucleotide	Sequence
1	5′ TTG TTC CCA TGT GAT TCC ACG 3′
2	5′ TTT TCC TCA TGT GCC ACT TTA 3′
3	5′ GTG TCC ATA TGT CCA CCT ACT 3′
4	5′ GAG AAC ACC ATA TGC TTG AGA 3′
5	5′ ATG GTA ATA TTG GAA TTC GTA TAA AA 3′
6	5′ CTG GCA CGC GTT GGA CGC GCA 3′
7	5′ TAT GTT AAC GGC GCG CCC ATG 3′
8	5′ GGG CGC GCC GTT AAC A 3′
9	5′ AAT TCA TAA AAC GAA AGG CTC AGT CGA AAG ACT GGG CCT TTC GTT TTA TCT GTT GTT TGC GGC CGC GGC CGC CTA GGC C 3′
10	5′ AGC TGG CCT AGG CGG CCG CGG CCG CAA ACA ACA GAT AAA ACG AAA ACG AAA GGC CCA GTC TTT CGA CTG AGC CTT TCG TTT TAT G 3′
11	5′ GGC GCG CCT GCA GAA TTC TCG AGA AGC TTC CCG GGA TCC TAG GCG CGC CAT GCA 3′
12	5′ TGG CGC GCC TAG GAT CCC GGG AAG CTT CTC GAG AAT TCT GCA GGC GCG CCC GCC 3′

^aOligonucleotides 1 to 6 were used as mutagenesis primers during site-directed mutagenesis (the mutagenic nucleotide[s] in each oligonucleotide is underlined). Oligonucleotides 7 to 12 were used for construction of the following polylinkers: oligonucleotides 7 and 8, NdeI-HpaI-AscI; oligonucleotides 9 and 10, EcoRI-rrnB T1-NotI-SfiI-kill HindIII; oligonucleotides 11 and 12, kill ApaI-AscI-PstI-EcoRI-XhoI-HindIII-SmaI-BamHI-AvrII-AscI-NsiI (Kill indicates that there were compatible cohesive ends which did not regenerate the restriction site indicated).