Table 2.
Effect of Variable Incubation Times at Room Temperature on Inactivation of Avian Paramyxovirus Subtype-1 (APMV-1) in Chicken Amnio-Allantoic Fluid (AAF) by MagMAX™ Lysis Buffer, as Measured by Hemagglutination Activity (HA) and Cycle Threshold (Ct) qRT-PCR Results.a
| 10-Minute Incubation Time | 20-Minute Incubation Time | 30-Minute Incubation Time | ||||||
|---|---|---|---|---|---|---|---|---|
| Egg No. | HA Result | qPCR Ct | Egg No. | HA Result | qPCR Ct | Egg No. | HA Result | qPCR Ct |
| 1 | – | No Ct | 1 | – | 35.93 | 1 | – | No Ct |
| 2 | – | No Ct | 2 | – | 35.6 | 2 | – | 35.58 |
| 3 | – | 35.77 | 3 | – | 34.89 | 3 | – | No Ct |
| 4 | – | No Ct | 4 | – | 34.84 | 4 | – | 37.89 |
| 5 | – | No Ct | 5 | – | 34.92 | 5 | – | No Ct |
| 6 | + | 14.62 | 6 | – | No Ct | 6 | – | No Ct |
| 7 | – | 22.15 | 7 | – | 37.24 | 7 | – | No Ct |
| 8 | – | 24.89 | 8 | – | 35.38 | 8 | – | No Ct |
| 9 | – | 24.11 | 9 | – | No Ct | 9 | – | No Ct |
| 10 | – | 23.32 | 10 | – | 36.9 | 10 | – | No Ct |
| 11 | – | No Ct | 11 | – | No Ct | 11 | – | No Ct |
| 12 | – | No Ct | 12 | – | No Ct | 12 | + | 13.2 |
| 13 | – | 39.84 | 13 | – | 36.37 | 13 | + | 14.35 |
| 14 | – | 35.15 | 14 | – | 0 | 14 | + | 12.92 |
| 15 | – | No Ct | 15 | + | 14.64 | 15 | + | 22.7 |
| Positive controlb (5) | All + | Average 14 | Positive control (5) | All + | Average 13.08 | Positive control (5) | All + | Average 12.6 |
| Negative control (3) | All – | No Ct | ||||||
a
Number of eggs used for controls in parentheses. One set of 3 eggs inoculated with viral transport media were included as negative controls across all time-series.
b
Result of positive control incubating for 10 minutes established in earlier trial (data not shown) and therefore not repeated to allow adequate positive controls for longer incubation times.