Figure 3.

Detection of trypsinated lysozyme in Aer^K238D+A260F. Plot of the event noise vs I_ex% with a representative current blockade for each event. The plot shows the data from three nanopores combined after baseline correction. Data were recorded in a mixture of 1 M KCl and 50 mM citric acid that was buffered to pH 3.0 using bis-tris propane at an applied potential of +150 mV. The sequences of the peptides are as follows: Lys4, WWC_mNDGR; Lys5, GTDVQAWIR; Lys6, GYSLGNWVC_mAAK; Lys7, FESNFNTQATNR; Lys8, IVSDGNGMNAWVAWR; and Lys9, NTDGSTDYGILQINSR. C_m indicates a cysteine alkylated using iodoacetamide (IAA).