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Physical map of the yeast YEpPGKαTDHβ high-copy-number plasmid containing the E. coli genes pntA and pntB, which encode the α and β subunits, respectively, of nicotinamide nucleotide transhydrogenase. Abbreviations: PGKp, PGK1 promoter, PGKt, PGK1 terminator; TDHp, TDH3 promoter, TDHt, TDH3 terminator. The directions of the promoter-gene-terminator fragments are the same in the pRSPGKαTDHβ low-copy plasmid.
