Fig. 7.

SEV-loaded miR-128 mimic potentiates decreased monocyte haptotaxis. A Schematic of workflow for SEV-miR-128 mimic studies. Briefly, monocytes were either transfected with miR-128 mimic or treated with HIV–COC– SEVs loaded with miR-128 mimic. Following 24 h incubation, cell viability was assessed, and cells were plated onto collagen I coated plates for haptotaxis assay. B Viability of monocytes treated with miR-128, SEV, or SEV-miR-128. C–D Gene expression for miR-128 target genes. E Representative plots of single monocyte tracks following treatment with miR-128, SEV, or SEVmiR-128. F–I Plots for monocyte directness, average velocity, kinetic distance migrated, and total distance migrated, respectively. The plots were calculated for 1 h at 9 equidistant fields of view per well, in triplicates. Ordinary one-way ANOVA test (Dunnett’s correction) was used to determine the differences between the treated groups as compared to vehicle treatment. Two-tailed Welch’s t test was used to compare the differences between SEV treatments. ****p < .001, ***p < .005, **p < .01, *p < .05, ns not significant