Fig 1.

Smoking-induced CBX3 upregulation is correlated with poor prognosis in pancreatic cancer. (A) Venn diagrams showed numbers of differentially expressed CBX family genes in current smokers compared to former smokers in TCGA-PAAD (Pancreatic adenocarcinoma), TCGA-LUAD (Lung adenocarcinoma) and TCGA-LUSC (Lung squamous cell carcinoma) datasets. (B) Box plot showed CBX3 was upregulated in current smokers compared to former smokers or never smokers in TCGA-PAAD dataset. (C and D) PANC-1 and BxPC-3 cells were treated with CSE (5%) for 0, 24, 48 and 72 hours as indicated, and then were collected for Western blot (C) and RT-qPCR assay (D). (E and F) PANC-1 and BxPC-3 cells were treated with a serial concentration of CSE for 72 hours, and then were collected for Western blot (E) and RT-qPCR assay (F). (G) The tissue-wise expression of CBX3 in Pancreatic adenocarcinoma (PAAD) tissues and non-tumor tissues were analyzed by the GEPIA web tool. P < 0.01*. (H) The mRNA and protein levels of CBX3 were examined. (I and J) CBX3-related Disease Free Survival (I) and Overall Survival (J) were determined by the GEPIA web tool. (K and L) CBX3-related Recurrence Free Survival (K) and Overall Survival (L) were checked in the ICGC-PACA-AU dataset. Data in (D), (F) and (H) are presented as mean ± SD (n = 3). P < 0.05 *; P < 0.01 **; P < 0.001 ***.