Fig 6.

The YBX1/CBX3 axis promotes tumor growth via suppressing SMURF2 in pancreatic cancer. (A-D) PANC-1 and SW1990 cells were transfected with indicated shRNAs for 72 hours. After puromycin selection, cells were harvested for the RT-qPCR (A), MTS (B), and colony formation (C and D). (E-G) PANC-1 cells were transfected with indicated shRNAs for 72 hours. After puromycin selection, cells were subcutaneously injected into the nude mice. The image of tumor was shown in panel E. The tumor mass and tumor growth curve were demonstrated in panel F and G, respectively. Data are presented as mean ± SD (n = 5). (H) PANC-1 and BxPC-3 cells were transfected with indicated shRNAs for 72 hours. After puromycin selection, cells were harvested for the Western blot analysis. (I) PANC-1 and BxPC-3 cells were transfected with indicated shRNAs for 48 hours, and then were transfected with indicated constructs for another 24 hours. Cells were harvested for Western blot. (J) A hypothetic model depicted that CSE exposure-induced YBX1 overexpression contributed to the upregulation of CBX3, which inhibited SMURF2 expression to promote the progression of pancreatic cancer. Data in (A), (B) and (D) are presented as mean ± SD (n = 3). Not significant ^Ns; P < 0.001 ***.