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Kinetics of induction of PsADH1 mRNA level upon shift to oxygen-limited conditions. Cells were grown on xylose under aerobic conditions (repressed state) until they reached an OD600 of 1.0, collected by centrifugation, and shifted to oxygen-limited conditions by suspension in the same medium. Aliquots of cells were taken at the indicated times, and total RNA was prepared for Northern analysis. Blots were probed with PsADH1.
