Skip to main content
. 2022 May 1;18(8):3209–3222. doi: 10.7150/ijbs.70289

Figure 3.

Figure 3

RCC-derived exosomes regulate macrophage polarization in vitro. A-B. Through flow cytometry, we found that compared with macrophages as the control group, the fluorescence intensity of CD163 and CD206 were significantly increased after RCC cell lines (789-O, ACHN and Caki-1) derived exosomes and macrophages co-cultivation (P<0.001) CD163:***P<0.001; CD206: ###P<0.001. C. The supernatants before and after the co-culture were collected, and the Elisa test showed that the expression of cytokines TGFbeta-1 and IL-10 increased, and IL-6, IL-12, IL-1beta decreased significantly after co-culture (p<0.05). D-E. The phagocytic ability of macrophages was significantly enhanced after co-culture with RCC-derived exosomes (p<0.05). F-G. Through tube formation assay we found that the pro-angiogenic ability of macrophages was significantly enhanced after co-cultured with RCC-derived exosomes (p<0.01).