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. 2022 May 1;18(8):3209–3222. doi: 10.7150/ijbs.70289

Figure 5.

Figure 5

Exosomal lncARSR could induce phenotypic and functional changes of macrophages in vitro. A. LncARSR plasmid/NC siRNA (control group)/lncARSR siRNA was transfected into macrophages, and we found that compared with the control group, the ability of cytokines secretion by macrophages with lncARSR plasmid transfection was enhanced (p<0.01), and on the contrary the ability of cytokines secretion by macrophages with lncARSR siRNA transfection was weakened through Elisa testing(p<0.01). B-C. The fluorescence intensity of CD163 and CD206 expressed by macrophages transfected with lncARSR plasmid was significantly enhanced (p<0.01), and the fluorescence intensity of CD163 and CD206 expressed by macrophages transfected with lncARSR siRNA was significantly decreased by flow cytometry (p<0.05). D-F. The phagocytic ability of macrophages transfected with lncARSR plasmid was enhanced (p<0.001), and inversely the phagocytic ability of macrophages transfected with lncARSR siRNA were suppressed (p<0.01). E-H. The proangiogenesis ability of macrophages was enhanced after lncARSR plasmid transfection (p<0.001) and inversely was inhibited by lncARSR siRNA through tube formation assay (p<0.05).