Figure 7.

Pharmacological inhibition of p38MAPK abrogates the promotion of lung metastasis by SNCG. (A) HepG2 cells stably transfected with empty vector (EV) or SNCG expression plasmid were injected into the mice tail veins, and then treated with or without SB203580 daily. The mice were sacrificed 42 days after injection. The sections from paraffin-embedded lung tissues were subjected to HE staining. The red arrows-indicated metastatic foci in representative lung sections were shown. Bars, 100 μm. (B) The area of metastic foci in lung sections was measured. The lung metastasis burden in each group was plotted. Bars, SEM. *, p < 0.05. (C) The phosphorylation of p38MAPK and expression of MKK3/6 and SNCG in lung micrometastases were detected by immunohistochemistry. Bar, 25 μm.