Figure 3.

m⁶A -seq analysis of cells in the M and G2 phases. (A)The eluted RNA and MeRIPed RNA from HeLa cells at M or G2 phases were analyzed by deep sequencing. Bar plot showing the numbers of m⁶A peaks and m⁶A -modified genes in the M and G2 phases. (B) Density distribution of the m⁶A peak across mRNA transcripts. m⁶A peak distribution in the 5′UTR, start codon, CDS, stop codon or 3′UTR region across the entire set of mRNA transcripts. (C) Regions of the 5′ untranslated region (5′UTR), coding region (CDS), and 3′untranslated region (3′UTR) were split into 100 segments, then percentages of m⁶A peaks that fall within each segment were determined. Pie chart showing the different m⁶A peak distribution between the 5'UTR, exon and 3'UTR regions. (D) Predominant consensus motif GGAC was detected in both G2 phase and M phase in m⁶A-seq. (E) Volcano plot showing the distribution of genes with differential m⁶A levels in the M phase groups compared with the G2 phase group. (F) Gene ontology (GO) analysis of genes with increased m⁶A methylation levels (Fold Change > 2; P < 0.05 in m⁶A-seq) in the M phase compared to the G2 phase. Gene counts of GO terms were exhibited on the x-axis and p-values were indicated with the color of columns.