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. 2022 May 9;18(8):3337–3357. doi: 10.7150/ijbs.69141

Figure 10.

Figure 10

PAD4 knockout protects mice against sepsis-associated ALI. (A) Paraffin-embedded lung tissue samples were stained for H&E. Representative histological images are shown at 400× magnification. (B) Pulmonary edema was evaluated by determining the wet/dry weight ratio in PAD4+/+ and PAD4-/- mouse models in the sham and sepsis-associated lung injury groups (n=6). (C) The cells in extracted BALF were analyzed by cell counting in PAD4+/+ and PAD4-/- mouse models in the sham and sepsis-associated lung injury groups (n=6). (D) Representative images of immunofluorescence staining of NETs in lung tissues from the sham and sepsis-associated lung injury murine models. (E) The cfDNA levels in plasma (n=6) and (F) BALF were detected in PAD4+/+ and PAD4-/- mouse models in the sham and sepsis-associated lung injury groups (n=6). (G) The levels of TNFα, IL-1α, IL-8 and TGF-β were determined by ELISA in PAD4+/+ and PAD4-/- mouse models in the sham and sepsis-associated lung injury groups (n=6). *P<0.05, **P<0.01 (Two-way ANOVA with Tukey's correction).