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. 2022 May 9;18(8):3337–3357. doi: 10.7150/ijbs.69141

Figure 2.

Figure 2

NETs formation inhibition, NETs depletion or NETs degradation protects mice against sepsis-associated ALI. A mouse model of cecal ligation and puncture (CLP)-induced sepsis-associated ARDS was established and then treated with saline, CI-amidine (inhibition of NETs formation), anti-ly6G (neutrophil depletion) or DNase (NETs degradation). (A) Paraffin-embedded mouse lung tissue samples were stained with H&E. Representative histological images are shown at 400× magnification. (B) Pulmonary edema was evaluated by determining the wet/dry weight ratio. (C) The cells in extracted bronchoalveolar lavage fluid (BALF) of mice were analyzed using a cell counter. (D) Representative images of immunofluorescence staining of NETs in lung tissues from HC, sham, and sepsis ARDS mouse models in the presence or absence of saline, CI-amidine, anti-ly6G and DNase (red: NE, green: CitH3, blue: DAPI). Scale bar=50 µm. (E) The cfDNA levels in plasma and (F) BALF were detected in the mouse model. (G) The levels of TNFα, IL-1α, IL-8 and TGF-β in plasma were determined by ELISA in a mouse model (n=6 in each group). *P<0.05. *indicates Sepsis-associated lung injury group versus HC group. # indicates Sepsis-associated lung injury group versus Sham group (Two-way ANOVA with Tukey's correction).