Figure 1: Loss of unc-30 disrupts the synaptic connectivity of the DD and VD MNs.
a: Schematic of DD rewiring 1.
b: Reconstruction of a VD4 MN from an unc-30(e191) adult animal compared to the same neuron in a wild-type animal. Extended Data Fig. 1 shows a more detailed presentation of the EM data.
c: Reconstructed DD3 neuron from an unc-30(e191) L1 larva showing aberrant NMJs in the dorsal cord (D). Previous reconstructions of a wild-type L1 using the same techniques and personnel showed that a reconstructed DD3 made no NMJs on dorsal muscles and 9 NMJs on ventral muscles 1.
d: Presynaptic marker RAB-3 ectopically localizes mostly to ventral cord in wild-type L1 animals (16/20 animals), but ectopically in the dorsal nerve cord (DNC; outlined in red) in unc-30 mutant animals (19/20). At the L4 stage, in which presynaptic specialization are observed in both ventral nerve cord (VNC; outlined in red) and DNC (19/20 animals), unc-30 mutants show few specializations in the VNC (20/20 animals).
E,F: Summary of synapse formation defects in unc-30, lin-14, and unc-55 mutants (E) and genetic interpretation (F).