Cholinergic innervation to the D-type MNs is visualized with an unc-47p::ACR-12::GFP reporter transgene, maintained in an acr-12(ok367) mutant background. 16.
a: ACR-12 puncta localization is affected by loss of lin-14. In wild-type L1 animals, ACR-12 puncta are observed only in the DD neurons in the dorsal (DNC) nerve cord (left). In lin-14 mutant L1 animals (scored in progeny from lin-14 null animals carrying a lin-14 rescue array 23), ACR-12 puncta are detected in the ventral nerve cord (VNC) of the DD MNs. Quantification of this data is represented in the graph. Some dorsal puncta in the DD MNs were still observed in 83% of the lin-14 mutant L1s that had puncta in the ventral nerve cord. L1 animals were obtained by hypochlorite-treating gravid adult animals and letting embryos hatch and arrest in M9 for 16-18 hours. n > 20 for each strain scored, **p < 0.01.
b: ACR-12 puncta localization is affected by loss of unc-55. In wild-type L4 animals, ACR-12 puncta are observed in both the ventral (VNC) and dorsal (DNC) nerve cords (left). In unc-55 mutant L4 animals, ACR-12 puncta are observed mostly in the ventral nerve cord of unc-55 mutants. Ventral and dorsal nerve cords are marked by red dotted lines. Quantification of this data is represented in the graph. n>20 for each strain, **p < 0.01.