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. Author manuscript; available in PMC: 2022 May 26.
Published in final edited form as: Nature. 2015 Jun 17;523(7558):83–87. doi: 10.1038/nature14545

Figure 2: Expression of oig-1 correlates with the inhibition of dorsal synapse formation and is controlled by unc-30, lin-14 and unc-55.

Figure 2:

a: oig-1 locus, oig-1 fosmid-based reporters (SL2::NLS::GFP fusion to assess gene regulation; GFP fusion after signal sequence to assess protein localization), deletion allele and oig-1fosmid::sl2::nls::gfp expression pattern (similar results with 2 independent lines).

b: oig-1 expression is regulated by the transcription factors unc-30 (0/20 animal express the reporter at any stage), lin-14 (3/20 L1 stage animals express the reporter but at reduced levels; scored in progeny from lin-14 null mothers carrying a lin-14 rescue array 23, n=20), and unc-55 (0/20 animal express the reporter in VD MNs; 20/20 animals express reporter in DD neurons), but not by alr-1 (20/20 animal express the reporter), in which VD identity but not synaptic wiring is affected 24. In lin-4 animals, oig-1 expression in DDs persists into the adult stage (20/20 adult animal express the reporter).