NPM1-ALK expressing SR-786, DEL, SUP-M2, SU-DHL-1, and Ba/F3-FG-NPM1-ALK cells were treated with indicated concentrations of gilteritinib for 24 hours. At the end of the treatment period, cell lysates were made, and Western blot analyses were performed for phospho-NPM1-ALK and total NPM1-ALK proteins. β-actin served as the loading control.