Fig 3. Stiffness specific gemcitabine resistance is not associated with altered levels of replication stress or DNA damage levels.

The MCF-7 cell line was cultured on 500 Pa and 4 kPa stiffness hydrogels for 24 hrs prior to treatment with 5 nM gemcitabine (Gem) or a vehicle control for a further 24 hrs. Cells were then fixed and stained using immunofluorescence for (A) RPA34 or (B) γH2AX. Representative images are shown for each stain. Nuclear intensity (integrated density) was measured in 100 nuclei for each of 3 independent repeats. The median and pooled data from the repeats are shown. Statistical significance was calculated using a Kruskal-Wallis test.