Table 4. Effect of supplementation of milk enriched with n-3 and n-6 on serum fatty acids profile of gilts from 34 to 190 days of age.

Fatty acidsa, μg.ml-1	Treatmentsb			P-valuec
	Control	n-3	n-6	Treatment	Time	Treat*Time	C1d	C2d
Myristic, C14:0	0.869 ± 0.070	0.669 ± 0.070	0.653 ± 0.070	0.087	<0.0001	0.062	0.031	0.875
Palmitic, C16:0	17.730 ± 0.700	17.800 ± 0.700	17.490 ± 0.070	0.950	0.0465	0.541	0.925	0.766
Stearic, C18:0	12.430 ± 0.300	11.830 ± 0.300	13.560 ± 0.300	0.006	<0.0001	0.408	0.497	0.002
Elaidic, C18:1t n-9	0.382 ± 0.080	0.293 ± 0.080	0.379 ± 0.080	0.686	0.0024	0.174	0.652	0.470
Palmitoleic, C16:1c9	1.136 ± 0.090	1.167 ± 0.100	1.117 ± 0.090	0.936	0.0016	0.937	0.961	0.721
Oleic, C18:1c9	23.330 ± 0.700	24.050 ± 0.700	22.130 ± 0.700	0.200	0.0028	0.684	0.790	0.081
Linoleic, C18:2 n-6	28.390 ± 0.500	29.770 ± 0.500	28.670 ± 0.600	0.225	0.0001	0.708	0.242	0.190
Dihomo-γ-linolenic, C20:3 n-6	0.695 ± 0.0400	0.688 ± 0.040	0.660 ± 0.050	0.852	<0.0001	0.637	0.714	0.679
ARA, C20:4 n-6	11.240 ± 0.500	11.000 ± 0.600	12.410 ± 0.600	0.239	<0.0001	0.587	0.534	0.121
γ-Linolenic, C18:3 n-6	0.506 ± 0.020	0.567 ± 0.030	0.531 ± 0.030	0.336	<0.0001	0.079	0.232	0.381
α-Linolenic, C18:3 n-3	0.563 ± 0.020	0.618 ± 0.030	0.588 ± 0.020	0.361	<0.0001	0.326	0.232	0.431
EPA, C20:5 n-3	0.219 ± 0.090	0.343 ± 0.090	0.461 ± 0.100	0.239	0.1157	0.280	0.143	0.397
DHA, C22:6 n-3	1.203 ± 0.100	1.184 ± 0.100	1.371 ± 0.100	0.459	<0.0001	0.300	0.617	0.260
Saturated	31.410 ± 0.300	30.370 ± 0.300	31.150 ± 0.300	0.090	<0.0001	0.450	0.124	0.106
Unsaturated	68.590 ± 0.300	69.630 ± 0.300	68.850 ± 0.300	0.090	<0.0001	0.450	0.124	0.106
SFA/USFA	0.458 ± 0.006	0.438 ± 0.006	0.453 ± 0.006	0.097	<0.0001	0.472	0.132	0.110
Monounsaturated	25.240 ± 0.600	25.430 ± 0.700	24.040 ± 0.600	0.312	0.0005	0.234	0.547	0.164
Polyunsaturated	43.340 ± 0.800	44.200 ± 0.800	44.880 ± 0.800	0.438	0.2194	0.229	0.255	0.563
Total n-3	2.002 ± 0.100	2.154 ± 0.100	2.384 ± 0.100	0.197	<0.0001	0.296	0.152	0.262
Total n-6	41.350 ± 0.700	42.050 ± 0.700	42.480 ± 0.800	0.596	0.0247	0.458	0.354	0.694
n-6/n-3	27.271 ± 1.100	26.347 ± 1.100	25.528 ± 1.100	0.559	<0.0001	0.459	0.341	0.612
ARA/EPA	59.854 ± 6.800	57.438 ± 6.800	46.762 ± 6.800	0.379	0.0043	0.174	0.368	0.285

^aEPA: Eicosapentaenoic acid; DHA: Docosahexaenoic acid; ARA: Arachidonic acid; USFA/SFA: Unsaturated/saturated fatty acids; Total n-3: Sum of n-3 fatty acids; Total n-6: Sum of n-6 fatty acids; n-6/n-3, ratio of total n-6 to total n-3 fatty acids; ARA/EPA: Ratio of arachidonic acid to eicosapentaenoic acid.

^bGilts fed with non-enriched milk (Control); or supplemented with cow’s milk enriched with n-3 or n-6.

^cC1, contrast between Control vs n-3+n-6; C2, contrast between n-3 vs n-6.