Table 1. Direct comparison of SARS-CoV-2 detection using RT-qPCR and extraction-free RT-LAMP.
| Samples | Purified Saliva RNA | Saliva Lysate | ||||||
|---|---|---|---|---|---|---|---|---|
| RT-qPCR N1 (Cq) | RT-qPCR N2 (Cq) | COVID LAMP (Tt) | COVID LAMP (Tt) | |||||
| 1 | 24.8 | 24.8 | 24.2 | 24.3 | 8.9 | 8.9 | 8.7 | 8.6 |
| 2 | 26.2 | 26.3 | 25.8 | 25.8 | 9.9 | 9.9 | 9.8 | 10.1 |
| 3 | 27.3 | 27.2 | 26.9 | 27.0 | 8.5 | 8.4 | 10.7 | 10.3 |
| 4 | 28.6 | 28.7 | 28.2 | 28.2 | 9.5 | 9.6 | 9.4 | 9.4 |
| 5 | 30.1 | 30.2 | 29.8 | 29.8 | 9.2 | 8.9 | 10.7 | 10.3 |
| 6 | 31.6 | 31.7 | 31.1 | 31.1 | 9.4 | 9.2 | 13.3 | 11.5 |
| 7 | 31.7 | 32.0 | 31.2 | 31.6 | 10.3 | 9.8 | 11.3 | 11.0 |
| 8 | 32.0 | 32.0 | 31.3 | 31.3 | 9.5 | 9.6 | 12.0 | 11.9 |
| 9 | 32.9 | 33.3 | 36.1 | 36.5 | 12.1 | 12.0 | 13.6 | 13.6 |
| 10 | 34.3 | 34.5 | 33.4 | 34.0 | 10.3 | 10.9 | 10.6 | 11.6 |
| 11 | 34.7 | 34.7 | N/A | N/A | 14.0 | 13.3 | 16.5 | 14.7 |
| 12 | 35.3 | 35.5 | 35.4 | 35.2 | 12.0 | 11.7 | 22.9 | N/A |
| 13 | 37.5 | N/A | N/A | N/A | N/A | N/A | 16.4 | N/A |
| 14 | 37.8 | 37.5 | 37.9 | 38.3 | 13.8 | 28.4 | N/A | N/A |
| 15 | 38.4 | 38.1 | N/A | N/A | N/A | 19.8 | N/A | N/A |
| 16 | 38.8 | 39.1 | N/A | N/A | N/A | N/A | N/A | N/A |
16 saliva samples containing a range of viral load were tested in RT-qPCR (N1 and N2 targets) and RT-LAMP(SARS-CoV-2). For RT-LAMP, both saliva lysate and purified RNA were used as input, whereas RT-qPCR was performed using only purified RNA. Cq values for RT-qPCR and Tt for RT-LAMP are shown. No amplification is denoted N/A.