TABLE 2.
Estimation of the major native molecular weights and pIs of major EH activities from A. alternata f. sp. lycopersici a
| Medium | Enzyme extract characteristic
|
||
|---|---|---|---|
| Molecular massb (Da) | pIbc | Ratio of 4.7 to 4.9 pI | |
| Pectin | |||
| Day 6 | |||
| Control | 20 ± 1 | 4.7 ± 1 | 4.7 |
| With clofibrated | 20 ± 1 | 4.7 ± 1 | 5.0 |
| Day 12 | |||
| Control | 20 ± 1 | 4.7 ± 1 | 3.9 |
| With clofibrated | 25 ± 1 | 4.9 ± 1 | 0.12 |
| Glucose, day 6 | |||
| pH 4 | 25 ± 1 | 4.9 ± 1 | 0.07 |
| pH 6 | 25 ± 1 | 4.9 ± 1 | 0.05 |
a
Only the characteristics of the major activity are given. Both the 20,000 and the 25,000 Da and the 4.7 and 4.9 pIs appear to be present in all the samples assayed.
b
Results are means ± standard deviations (n = 3).
c
Yields in activity between 30 and 40% were obtained.
d
Added at 1 mM.