Table 2.
Serum parameters of adult male and female wild type (WT, C57BL/6J) and Pxr-null mice and after 16 weeks on a control or a high-fat diet (HFD).
| Serum Parameter | Male WT Control (n = 7) | HFD (n = 9) | Female WT Control (n = 7) | HFD (n = 8) | Male PXR-KO Control (n = 11) | HFD (n = 12) | Female PXR-KO Control (n = 11) | HFD (n = 11) |
|---|---|---|---|---|---|---|---|---|
| ALT (IU/L) | 27.1 ± 4.8 | 82.6 ± 9.0# | 21.7 ± 4.1 | 35.1 ± 11.5β | 27.7 ± 2.6 | 22.6 ± 4.7† | 24.7 ± 5.8 | 36.4 ± 17.5 |
| Bile Acids (μmole/L) | 37.3 ± 2.4 | 62.1 ± 4.2# | 32.2 ± 2.0 | 33.5 ± 3.7β | 35.6 ± 1.7 | 42.1 ± 7.0† | 34.8 ± 3.7 | 35.6 ± 4.0 |
| FGF21 (pg/mL) | 764 ± 84 | 1343 ± 187# | 275 ± 85 | 614 ± 125 β | 225 ± 74 | 356 ± 69† | 337 ± 92 | 453 ± 86 |
| Adiponectin (ng/mL) | 11.1 ± 0.7 | 9.4 ± 0.4 | 17.0 ± 0.4‡ | 18.3 ± 0.7β | 7.7 ± 0.7 | 7.4 ± 0.6 | 16.2 ± 1.8‡ | 16.0 ± 0.8β |
| Insulin (pg/mL) | 2.4 ± 0.7 | 19.5 ± 2.1# | 1.7 ± 0.6 | 2.3 ± 0.8β | 0.9 ± 0.4 | 14.4 ± 5.5# | 0.7 ± 0.1 | 1.1 ± 0.2β |
| Leptin (ng/mL) | 31.2 ± 3.0 | 48.1 ± 2.2# | 18.1 ± 2.5 | 51.6 ± 4.8# | 12.9 ± 3.1* | 35.4 ± 4.0# | 16.4 ± 3.1 | 38.7 ± 4.4# |
| Fasting glucose (mg/dL) | 133 ± 15 | 170 ± 11 | 117 ± 11 | 137 ± 8 | 119 ± 12 | 191 ± 11# | 108 ± 10 | 130 ± 6β |
Data represent mean ± SEM for 7–11 mice per group.
§
P < 0.05 between female mice fed HFD.
1
For body mass composition, n = 3–4 and were performed after 3 month of diet administration
‡
P < 0.05 between male and female mice fed the control diet.
#
P < 0.05 between mice fed control diets and high-fat diet (HFD).
†
P < 0.05 between male mice fed HFD.
β
P < 0.05 between male and female mice fed a HFD.