Fig 9. A. marginale-specific IgG response in cattle.

Antigen-specific IgG antibodies were measured by ELISA in plasma samples collected from day zero (prior to infection) and multiple time points post vaccination and post challenge. Purified A. marginale whole cell antigens recovered from ISE6 cell cultures were used to coat the ELISA plates. Average absorbance values of plasma collected from steers within each group were plotted against plasma collection days. At, significance level α = 0.05, One-way ANOVA with repeated measures showed that IgG levels were significantly different between the infection control, WCAV and MLAV groups (P < 0.0001****), for several days post-challenge. Additionally, Tukey’s multiple comparisons test showed significant differences between infection control and WCAV groups (P = 0.0003***), infection control and MLAV groups (P = 0.0024**), and WCAV and MLAV groups (P = 0.0041**).