Skip to main content
. 2022 May 16;18(5):e1010345. doi: 10.1371/journal.ppat.1010345

Fig 2. iRNA knockdown of Hh signaling components inhibits E. chaffeensis infection.

Fig 2

(A) Small interfering RNA-transfected (siRNA) THP-1 cells were infected or mock infected (-) with E. chaffeensis (MOI 100, 24 h post-transfection). Scrambled siRNA (scrRNA) was transfected for positive (infected) and negative control (mock infected). E. chaffeensis infection was quantified at 24 hpi and was determined by qPCR amplification of the dsb gene. siRNA knockdown (KD) of Hh receptors PTCH2, SMO, and transcription factors GLI-1/2/3 significantly inhibits E. chaffeensis infection in THP-1 cells. All knockdowns were performed with at least three biological and technical replicates for t-test analysis. Data are represented as means ± SD (***p<0.001). (B) Western blot depicts knockdown efficiency of siRNA in knockdown cells compared to positive control from whole-cell lysates harvested 24 hpi. Number left of siRNA lane indicates percent knockdown of protein of interest relative to positive control, normalized to GAPDH expression.