Figure 5.

Stimulation of ILC2 cells with type I interferons reduces proliferation, viability and cytokine production. (a) Proliferation of sorted and expanded BM-derived wild-type ILC2 cells labeled with the division-tracking dye CFSE and then stimulated for 3 or 5 d (above plots) with medium or IL-7 plus IL-33 in the presence or absence of IFN-β (as in Fig. 2d), analyzed by flow cytometry. (b) Proliferation of sorted and expanded BM-derived wild-type ILC2 cells stimulated for 2 or 3 d (left margin) as in a, assessed by staining with annexin V and viability dye (LIVE/DEAD), followed by flow cytometry. (c) Expression of IL-5 and IL-13 by sorted and expanded BM-derived wild-type ILC2 cells stimulated for 3 d as in a, assessed by intracellular flow cytometry. (d) Quantification of IL-5⁺ and IL-13⁺ cells and intracellular staining of IL-5 and IL-13 (presented as mean fluorescence intensity relative to that of isotype-matched control antibody), for cells as in c. Numbers in quadrants (b,c) indicate percent cells in each. *P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001 (Mann-Whitney test). Data are representative of two independent experiments (mean and s.d. in d).