Table 2.
Primer sequences of virulence genes and PCR conditions used in this study.
| Target gene | Primer | Primer sequence (5′ ⟶ 3′) | Conditions | Cycles | Size (bp) | References |
|---|---|---|---|---|---|---|
| cdtA | CDTA-FCDTA-R | CCTTGTGATGCAAGCAATC ACACTCCATTTGCTTTCTG | 94°C for 15 min, 94°C for 1 min, 49°C for 1 min, and 72°C for 1 min, 72°C for 7 min | 45 | 370 | [39] |
|
| ||||||
| cdtB | CDTB-FCDTB-R | GTTAAAATCCCCTGCTATCAACCA GTTGGCACTTGGAATTTGCAAGGC | 94°C for 15 min, 94°C for 1 min, 51°C for 1 min, and 72°C for 1 min, 72°C for 7 min | 45 | 495 | [39] |
|
| ||||||
| cdtC | CDTCFCDTCR | CGATGAGTTAAAACAAAAAGATA TTGGCATTATAGAAAATACAGTT | 94°C for 15 min, 94°C for 1 min, 48°C for 1 min, and 72°C for 1 min, 72°C for 7 min | 45 | 182 | [39] |
|
| ||||||
| cadF | cadF-F2BcadF-R1B | TTGAAGGTAATTTAGATATGCTAATACCTAAAGTTGAAAC | 95°C for 3 min, 94°C for 30 s, for 30 s, 43°C and 72°C for 1 min, 72°C for 5 min | 45 | 400 | [10] |
|
| ||||||
| ciaB | CIAB-652CIAB R1159 | TGCGAGATTTTTCGAGAATGTGCCCGCCTTAGAACTTACA | 95°C for 3 min, 94°C for 30 s, for 30 s, 54°C and 72°C for 1 min, 72°C for 5 min | 45 | 527 | [38] |
|
| ||||||
| pldA | PLDA-FPLDA-R | AAGAGTGAGGCGAAATTCCAGCAAGATGGCAGGATTATCA | 95°C for 3 min, 94°C for 30 s, for 30 s, 46°C and 72°C for 1 min, 72°C for 5 min | 45 | 385 | [38] |