Figure 2.

Overexpression of miR-4735-3p induces ferroptosis of human ccRCC cell lines. (a) Relative expression of miR-4735-3p in human ccRCC cell lines treated with the miR-4735-3p mimic or mimic control. (b) Cell viability was examined by the MTT method. (c) Relative LDH level in the medium from the miR-4735-3p mimic or mimic control-treated ccRCC cells. (d and e) Relative ROS and MDA levels. (f and g) Relative intracellular iron and Fe²⁺ levels. (h) Cell viability in the miR-4735-3p mimic-treated ccRCC cells in the presence of various cell death inhibitors. N = 6 per group. All data were expressed as the mean ± SD; ^∗P < 0.05 was considered significant.