Figure 10.
Nucleic acid detection with SERS or fluorescent sensors. (a) “Three-strand” hybridization assay using SERS probes for detection of Hepatitis B Virus DNA. (b) “Two-strand” hybridization mode-based competitive interaction between single-strand and double-stranded complex graphene oxide. (c) “Molecular beacon” mode for detection of influenza virus RNA. (d) Nucleic acid amplification and test (NAAT) using self-circulation of chain reaction for signal amplification and “molecule beacon” mode for detection. (e) Taq polymerase-assisted assay. (f) CRISPR/Cas12a assisted assay with fluorescence probes determined by the size of metal nanoparticles and their distance. Reproduced with permission from (a) ref. 112, copyright 2013, American Chemical Society; (b) ref. 132, copyright 2013, American Chemical Society; (c) ref. 136, copyright 2016, Elsevier; (d) ref. 135, copyright 2019, The Royal Society of Chemistry; (e) ref. 137, copyright 2012, The Royal Society of Chemistry; (f) ref. 138, copyright 2020, American Chemical Society.