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Comparison of total cell counts determined by visual microscopy and by flow cytometry. Cells were incubated with CTC and then stained with PicoGreen for flow cytometry and with DAPI for visual microscopy. The coefficients of variation for the visual counts (two replicate slides) ranged from 1 to 33% (mean, 13%), and coefficients of variation for flow cytometry (three replicates) ranged from 2 to 8% (mean, 3.9%).
