TABLE 2.
Percentages of CTC-active cells after different treatments with the reducing agent SDT
| SDT concn (mM) | % of CTC-active cells
|
||||||
|---|---|---|---|---|---|---|---|
| Preserved cellsa
|
Live cellsb
|
||||||
| Expt 2 (unenriched) | Expt 3 (enriched) | Expt 4 (enriched) | Expt 5 (unenriched) | Expt 2 (unenriched) | Expt 3 (enriched) | Expt 4 (enriched) | |
| 0 (control)c | 6 | 12 | 12 | 10 | 6 | 12 | 12 |
| 5 | 6 | 20 | 29 | 6 | 43 | 7 | |
| 50 | 7 | 24 | 21 | 8 | 9 | 15 | 19 |
| 100 | 22 | 15 | 24 | ||||
| 300 | 14 | ||||||
| 500 | 32 | 14 | 41 | ||||
a
Cells were fixed with 1% paraformaldehyde after the CTC treatment and before rinsing and the SDT treatment.
b
Cells were fixed after the CTC and SDT treatments.
c
Data obtained when the regular CTC technique was used.