Figure S3.

Multifunctional spike-specific CD4⁺ T cells expressing iCD40L⁺ in subjects vaccinated with the mRNA-1273, BNT162b2, Ad26.COV2.S, or NVX-CoV2373 COVID-19 vaccines. Related to Figure 4

(A) Comparison of multifunctional profiles of spike-specific CD4⁺ T cells iCD40L⁺Secreted-effector⁺ in subjects vaccinated with the mRNA-1273, BNT162b2, Ad26.COV2.S, or NVX-CoV2373 COVID-19 vaccine at T2, T3, T4, and T5. The blue, green, yellow, and red colors in the stacked bar charts depict the production of one, two, three, and four Secreted-effector⁺ functions, respectively. Data were analyzed for statistical significance using the Kruskal-Wallis (KW) test and Dunn’s post-test for multiple comparisons.

(B) Predominant multifunctional profiles of spike-specific CD4⁺ T cells expressing iCD40L with one, two, three, and four Secreted-effector⁺ functions were analyzed in subjects vaccinated with the mRNA-1273, BNT162b2, Ad26.COV2.S, or NVX-CoV2373 COVID-19 vaccine at 6 months post-vaccination (T5). Boolean analysis was carried out to define the functional profiles and the analysis included GzB, IFNγ, IL-2, and TNFα gated on CD3⁺CD4⁺ cells expressing iCD40L (See Figure 4). Each Secreted-effector⁺ profile combination was considered positive with >0.005% and an SI > 2 for CD4⁺ T cells. The dotted line indicates the limit of quantification (LOQ). The bars show the Geometric mean and geometric SD of the spike-specific CD4⁺ T cells iCD40L⁺.