TABLE 2.
Description of morphological and molecular characteristics, classes, and variables used for UPGMA and CA of the T. viride data set
| Characteristic | Characteristic described | Class | Variable |
|---|---|---|---|
| 1 | Angle subtending the phialide (A) | 1 = >100° | A1 |
| 2 = <100° | A2 | ||
| 3 = unknown | A3 | ||
| 2 | Length of conidia from CMD (CL) | 1 = <3.9 μm | CL1 |
| 2 = >3.9 μm | CL2 | ||
| 3 = unknown | CL3 | ||
| 3 | Width of conidia from CMD (CW) | 1 = <3.1 μm | CW1 |
| 2 = >3.1 μm | CW2 | ||
| 3 = unknown | CW3 | ||
| 4 | Length of conidia from SNA (SL) | 1 = 2.5–3.5 μm | SL1 |
| 2 = 3.6–4.0 μm | SL2 | ||
| 3 = >4.0 μm | SL3 | ||
| 4 = unknown | SL4 | ||
| 5 | Width of conidia from SNA (SW) | 1 = 2.5–3.0 μm | SW1 |
| 2 = 3.1–3.5 μm | SW2 | ||
| 3 = >3.5 μm | SW3 | ||
| 4 = unknown | SW4 | ||
| 6 | Phialide length (PH) | 1 = <8.2 μm | PH1 |
| 2 = 8.2–8.5 μm | PH2 | ||
| 3 = >8.5 μm | PH3 | ||
| 7 | Phialide middle (M) | 1 = 2.0 to <3.0 μm | M1 |
| 2 = >3.0 μm | M2 | ||
| 8 | Phialide base (B) | 1 = <2.0 μm | B1 |
| 2 = >2.0 and <2.9 μm | B2 | ||
| 3 = >2.9 μm | B3 | ||
| 9 | Hypha below phialide, axis (AX) | 1 = <2.0 μm | AX1 |
| 2 = 2.0–2.9 μm | AX2 | ||
| 3 = >2.9 μm | AX3 | ||
| 10 | Chlamydospore diameter (CY) | 1 = <9.0 μm | CY1 |
| 2 = 9–9.5 μm | CY2 | ||
| 3 = >9.5 μm | CY3 | ||
| 4 = unknown | CY4 | ||
| 11 | l/w ratio of phialides (PR) from CMD | 1 = <3.0 | PR1 |
| 2 = >3.0 | PR2 | ||
| 3 = unknown | PR3 | ||
| 12 | l/w ratio of conidia (CR) from CMD | 1 = 1.0–1.2 | CR1 |
| 2 = 1.3–1.7 | CR2 | ||
| 3 = unknown | CR3 | ||
| 13 | Ascus length (AL) | 1 = <85 μm | AL1 |
| 2 = >85 μm | AL2 | ||
| 3 = unknown | AL3 | ||
| 14 | Ascus width (AW) | 1 = <6.0 μm | AW1 |
| 2 = >6.0 μm | AW2 | ||
| 3 = unknown | AW3 | ||
| 15 | Length, distal part, ascospore (DL) | 1 = <4.5 μm | DL1 |
| 2 = >4.5 μm | DL2 | ||
| 3 = unknown | DL3 | ||
| 16 | Width, distal part, ascospore (DW) | 1 = <3.9 μm | DW1 |
| 2 = >3.9 μm | DW2 | ||
| 3 = unknown | DW3 | ||
| 17 | Length, proximal part, ascospore (XL) | 1 = 4.0–4.9 μm | XL1 |
| 2 = >4.9 μm | XL2 | ||
| 3 = unknown | XL3 | ||
| 18 | Width, proximal part, ascospore (XW) | 1 = <3.6 μm | XW1 |
| 2 = >3.6 μm | XW2 | ||
| 3 = unknown | XW3 | ||
| 19 | Stromal diameter (ST) | 1 = <1.5 mm | ST1 |
| 2 = >1.5 mm | ST2 | ||
| 3 = unknown | ST3 | ||
| 20 | Perithecial height (PI) | 1 = <232 μm | PI1 |
| 2 = >232 μm | PI2 | ||
| 3 = unknown | PI3 | ||
| 21 | Perithecial width (PD) | 1 = 140–170 μm | PD1 |
| 2 = 171–180 μm | PD2 | ||
| 3 = >180 | PD3 | ||
| 4 = unknown | PD4 | ||
| 22 | Width of surface layer of stroma (SR) | 1 = <20 μm | SR1 |
| 2 = >20 μm | SR2 | ||
| 23 | Diameter of surface cells of stroma seen in section (SC) | 1 = 4.5–5.0 μm | SC1 |
| 2 = >5.0 μm | SC2 | ||
| 3 = unknown | SC3 | ||
| 24 | Surface cells of stroma seen in surface view (SV) | 1 = <5.0 μm | SV1 |
| 2 = 5.0–5.5 μm | SV2 | ||
| 3 = >5.5 μm | SV3 | ||
| 4 = unknown | SV4 | ||
| 25 | Cells of interior of stroma below perithecia (IN) | 1 = 5.0–6.0 μm | IN1 |
| 2 = 6.1–7.0 μm | IN2 | ||
| 3 = >7.0 μm | IN3 | ||
| 26 | Hairs on stroma surface, length (HL) | 1 = <12 μm | HL1 |
| 2 = >12 μm | HL2 | ||
| 3 = unknown | HL3 | ||
| 27 | Hairs on stroma surface, width (HW) | 1 = 3.5–4.1 μm | HW1 |
| 2 = >4.1 μm | HW2 | ||
| 3 = unknown | HW3 | ||
| 28 | Conidial shape (CT) | 1 = subglobose, strongly warted | CT1 |
| 2 = subglobose, weakly warted | CT2 | ||
| 3 = subglobose, smooth | CT3 | ||
| 4 = oblong to ellipsoidal, smooth | CT4 | ||
| 29 | Presence of coconut odor (OD) | 1 = absent | OD1 |
| 2 = present | OD2 | ||
| 30 | Formation of chlamydospores on CMD within 1 week (CH) | 1 = present | CH1 |
| 2 = absent | CH2 | ||
| 31 | Growth on PDA at 72 h, 25°C (P25) | 1 = weak growth | P251 |
| 2 = moderate growth | P252 | ||
| 3 = strong growth | P253 | ||
| 32 | Growth on PDA at 72 h, 30°C (P30) | 1 = weak growth | P301 |
| 2 = moderate growth | P302 | ||
| 3 = strong growth | P303 | ||
| 4 = no growth | P304 | ||
| 33 | Time (h) at which conidia first observed on PDA at 25°C (PF) | 1 = 32 h | PF1 |
| 2 = 40 h | PF2 | ||
| 3 = 64 h | PF3 | ||
| 4 = 72 h | PF4 | ||
| 5 = conidia not formed | PF5 | ||
| 34 | Time (h) at which conidia first observed on SNA at 25°C (SF) | 1 = 32 h | SF1 |
| 2 = 40 h | SF2 | ||
| 3 = 64 h | SF3 | ||
| 4 = 72 h | SF4 | ||
| 5 = conidia not formed | SF5 | ||
| 35 | Conidial color at 25°C on PDA (CC) | 1 = green | CC1 |
| 2 = pale green | CC2 | ||
| 3 = white | CC3 | ||
| 4 = conidia not formed | CC4 | ||
| 36 | Colony reverse on PDA (PS) | 1 = cream | PS1 |
| 2 = cream and folded | PS2 | ||
| 3 = none | PS3 | ||
| 37 | Margin of colony on SNA, 10 days (SE) | 1 = smooth | SE1 |
| 2 = lobed | SE2 | ||
| 3 = fringed, thick ends | SE3 | ||
| 4 = fringed | SE4 | ||
| 5 = loose | SE5 | ||
| 6 = none | SE6 | ||
| 38 | Colony radius on PDA, 25°C, 24 h (P3) | 1 = <5 mm | P31 |
| 2 = >5–10 mm | P32 | ||
| 3 = >10–15 mm | P33 | ||
| 4 = >15 mm | P34 | ||
| 39 | Colony radius on SNA, 25°C, 48 h (S3) | 1 = <5 mm | S31 |
| 2 = <5–10 mm | S32 | ||
| 3 = >10–20 mm | S33 | ||
| 4 = >20–25 mm | S34 | ||
| 5 = >25 mm | S35 | ||
| 40 | Surface of aggregate on CMD (AS) | 1 = conidia formed at surface; projecting, terminally fertile conidiophores conspicuous | AS1 |
| 2 = conidia formed from plumose, projecting conidiophores that are entirely fertile | AS2 | ||
| 3 = aggregates uniformly cottony with no projecting conidiophores | AS3 | ||
| 41 | Morphology of phialides (PM) | 1 = hooked and/or sinuous phialides present | PM1 |
| 2 = phialides straight | PM2 | ||
| 3 = unknown | PM3 | ||
| 42 | Morphology of branches (BT) | 1 = regular with branches tending to be paired and regularly spaced | BT1 |
| 2 = irregular with long internodes between branches, fertile aggregate tending to be sinuous; phialides tending to be solitary | BT2 | ||
| 3 = unknown | BT3 | ||
| 43 | RAPD pattern M13 (RAPD) | Patterns 1–8 | RAPD1–RAPD8 |
| No data | RAPD9 | ||
| 44 | RAPD pattern (GACA)4 (TYPE) | Patterns 1–8 | TYPE1–TYPE8 |
| No data | TYPE9 | ||
| 45 | ITS sequence type (ITS) | Types 1–8 | ITS1–ITS8 |
| 46 | Endochitinase gene fragment (EGF) | 1 = 1 fragment | EGF1 |
| 2 = 2 fragments | EGF2 | ||
| 3 = unknown | EGF3 | ||
| 47 | Endochitinase gene RFLP, HaeIII (EH) | Patterns 1–12 | EH1–EH12 |
| No data | EH13 | ||
| 48 | Endochitinase gene RFLP, HhaI (HhaI) | Patterns 1–6 | HhaI1–HhaI6 |
| No data | HhaI7 | ||
| 49 | Endochitinase gene RFLP, MspI (MspI) | Patterns 1–11 | MspI1–MspI11 |
| No data | MspI12 | ||
| 50 | Endochitinase gene RFLP, Sau96I (Sau96I) | Patterns 1–5 | Sau96I1–Sau96I5 |
| No data | Sau96I6 | ||
| 51 | 28S sequence type (D28) | Types 1–6 | D281–D286 |
| No data | D280 | ||
| 52 | 28S RFLP, HaeIII (DH28) | Pattern 1 | DH281 |
| No data | DH280 | ||
| 53 | 28S RFLP, HhaI (RHh) | Pattern 1 | RHh1 |
| No data | RHh0 | ||
| 54 | 28S RFLP, MspI (PM) | Pattern 1 | PM1 |
| Pattern 2 | PM2 | ||
| No data | PM0 | ||
| 55 | 28S RFLP, Sau96I (PS) | Pattern 1 | PS1 |
| No data | PS0 |