Table 1.
Combination of anti-PyCSP and anti-PyTRAP can improve sterile protection from mosquito-bite challenge.
| Sterile protectiona Exp 1 | Sterile protectiona Exp 2 | Sterile protectiona Exp 3 | Sterile protectiona Combined | Comparison p valueb vs. 400 µg mIgG | Comparison p valueb vs. 100 µg αCSP + 300 µg mIgG | |
|---|---|---|---|---|---|---|
| 400 µg mIgGc | 1/5 (20%) | 0/5 (0%) | 1/5 (20%) | 2/15 (13.3%) | – | 0.033 |
| 100 µg αCSP + 300 µg mIgGc | 4/8 (50%) | 3/5 (60%) | 2/5 (40%) | 9/18 (50%) | 0.033 | – |
| 300 µg TY11 + 100 µg mIgGc | 1/3 (33%) | 0/5 (0%) | 1/5 (20%) | 2/13 (15.4%) | 0.956 | 0.049 |
| 300 µg TY12 + 100 µg mIgGc | 2/4 (50%) | 0/5 (0%) | 0/4 (0%) | 2/13 (15.4%) | 0.956 | 0.049 |
| 300 µg TY03 + 100 µg mIgGc | 5/6 (83.3%) | 0/5 (0%) | 2/5 (40%) | 7/16 (43.7%) | 0.072 | 0.734 |
| 100 µg αCSP + 300 µg TY11 | 2/4 (50%) | 1/5 (20%) | 1/5 (20%) | 4/14 (28.6%) | 0.355 | 0.279 |
| 100 µg αCSP + 300 µg TY12 | 3/4 (75%) | 0/5 (0%) | 2/5 (40%) | 5/14 (35.7%) | 0.211 | 0.586 |
| 100 µg αCSP + 300 µg TY03 | 6/6 (100%) | 4/5 (90%) | 4/5 (80%) | 14/16 (87.5%) | 0.000034 | 0.025 |
aSterile protection: mice that remain parasite-free (via microscopic blood-smear monitoring) throughout the experimental time course.
bBarnard’s exact test p values shown were not adjusted for multiple comparisons due to small group sizes and a small number of predefined comparisons being made.
cmIgG: normal mouse IgG control
Mice were injected with 100 μg/mouse of anti-CSP mAb (2F6), 300 μg/mouse of an anti-PyTRAP mAb, or a combination of both 24 h prior to challenge by five Py-infected mosquitoes. Where only one mAb was injected, mice were also given nonspecific mIgG to a total of 400 µg/mouse. Mice were tracked for 14 days for parasitemia by thin blood smear and those remaining parasite-free at day 14 were considered sterilely protected. The number and percentages of mice protected across three independent experiments are shown.