Fig. 5. P. falciparum midgut infection is restored by plasmin supplementation.

a–c WT and transgenic mosquitoes were fed by SMFA with P. falciparum infected blood supplemented with plasma plus increasing concentrations of plasmin. Horizontal red lines represent the median oocyst number of data pooled from three independent experiments shown in Supplementary Data 1, Dataset 6. Statistical analysis was done by Kruskal–Wallis multiple comparisons with Dunn’s posttest, ****P < 0.001, ns not significant. I inhibition, N number of analyzed mosquitoes. The percent inhibition of median, and prevalence was calculated as follows: 100 × [(number of oocysts in the control − number of oocysts in the experimental)/(number of oocysts in the control)].