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. 2022 May 26;13:2950. doi: 10.1038/s41467-022-30646-4

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2022

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Fig. 2 — Schematic of CITE-seq approach using hashtag antibodies (Abs) and Total-SeqC antibodies. b Unbiased clustering of 33,322 single cells labeled broadly by cell type category and colored by high-resolution cell type identities via Uniform Manifold Approximation and Projection (UMAP). Populations include lipid-associated macrophages (LAMs), conventional dendritic cells (cDCs), plasmacytoid DCs (pDCs), monocyte-derived DCs (moDCs), T helper (Th) cells, T regulatory cells (Tregs), gamma-delta (gd) T cells, natural killer (NK) cells, and type 2 innate-like lymphoid cells (ILC2s). Selected markers of specific cell subsets based on c gene expression and d surface protein. e Phylogenetic tree of high-resolution cell type identities. Figure 2a was created with Biorender.com.