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. 2022 May 26;13:2950. doi: 10.1038/s41467-022-30646-4

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2022

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Fig. 6 — a Uniform Manifold Approximation and Projection (UMAP) of macrophage subclusters plotted by diet groups for lean, obese, weight loss (WL), and weight cycled (WC) mice. Populations include lipid-associated macrophages (LAMs). b Expression levels for genes (Lyz2, Cst3, Adgre1, Cd68, Lgals3, and Itgam) and proteins (CITE-seq; MAC2, CD64, and CD11b) associated with macrophages. c Expression of genes that associate with macrophage subclusters. d Counts per hundred cells sequenced for macrophage subclusters (mean ± SEM; n = 4 mice). Box indicates interquartile range (25th–75th percentile) with 50th percentile indicated by solid line and mean indicated by large circle. Range of whiskers indicates largest and smallest values within 1.5 times the interquartile range and values outside of the range are indicated by small circles. e UMAP visualization of Mrc1 and Cd163 expression in tissue resident macrophages (TRMs) by diet group. f Embedding of RNA velocity displayed on the UMAP for macrophage and monocyte subsets. g TRMs and LAMs plotted on based on the macrophage polarization index (MPI) and the activation-induced macrophage differentiation index (AMDI) calculated using MacSpectrum. A shift to the right indicates a more M1-like phenotype. For diet groups, gray = lean, blue = obese, green = WL, orange = WC. Each macrophage subset indicated with a different shade of blue. Pairwise two-tailed t-tests with Bonferroni correction for multiple comparisons were used to compare groups for cell counts; significant p values shown.