Fig. 6. Sox2 overexpression in adult pancreatic epithelial cells drives acinar-ductal metaplasia.

a Whole-mount images of Mist1^+/+;R26^{Sox2-IRES-GFP/+} (n = 3, left) and Mist1^CreERT2/+;R26^{Sox2-IRES-GFP/+} (n = 3, right). Scale bar = 5 mm, yellow dotted lines outline the pancreas. b H&E staining of Mist1^+/+;R26^{Sox2-IRES-GFP/+} (left) and Mist1^CreERT2/+;R26^{Sox2-IRES-GFP/+} (right) pancreas tissue. Scale bars are 100 µm for top panels and 50 µm for bottom panels. Black dashed box represents the magnified area. Immunofluorescence staining of Mist1^+/+;R26^{Sox2-IRES-GFP/+} (left) and Mist1^CreERT2/+;R26^{Sox2-IRES-GFP/+} (right) pancreas for c SOX2 (red) and GFP (green), d Amylase (red) and GFP (green), e MUC1 (red) and GFP (green), and f Ki67 (red) and GFP (green). Scale bars for (a–f) are 50 µm. g Mean (±SEM) percent of Ki67 positive cells in Mist1^+/+;R26^{Sox2-IRES-GFP/+} (left, n = 3) and Mist1^CreERT2/+;R26^{Sox2-IRES-GFP/+} (right, n = 3) pancreas tissue (*P = 0.0124, by two-sided, unpaired t-test with Welch’s correction). Source data are provided as a source data file.