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. 2022 May 26;13:2958. doi: 10.1038/s41467-022-30620-0

Fig. 1. The adipocyte-specific lncRNA ADIPINT (CATG00000106343.1) is linked to changes in fat mass and fat cell volume.

Fig. 1

a Identification of differentially-expressed lncRNAs in white adipose tissue (WAT) two (n = 49) and five (n = 38) years post-Roux-en-Y gastric bypass (RYGB) surgery compared to pre-RYGB (n = 50). The five lncRNA with the greatest decrease in fold change at two and five years are listed, including ADIPINT (CATG00000106343.1). b Correlation of the change in ADIPINT expression versus the change in total fat mass or adipocyte cell volume from zero to two, zero to five and two to five years post-RYGB. Each data point represents an individual patient. Linear regression was used to assess significance with no adjustment for multiple comparisons. c Genomic position of ADIPINT on chromosome 9 is indicated and the expression detected by RNA-Seq and CAGE-Seq in human adipose-derived stem cells (hADSC) at day 14 of differentiation and the full length transcript determined by PacBio-Seq is shown. The conservation of the ADIPINT genomic region in mammalian and non-mammalian species is displayed above. d qRT-PCR analysis of ADIPINT expression in fractionated mature adipocytes (MA) and stroma-vascular fraction cells (SVF) from human WAT, n = 10 independent samples. e qRT-PCR analysis of ADIPINT during hADSC differentiation into adipocytes, n = 3 independent experiments. One-way ANOVA with Dunnett’s post-hoc test was used to assess significance compared to day 0 of differentiation and one-way ANOVA with Holm-Sidak’s post-hoc test was used to assess significance between WAT fractionation samples. f Tissue distribution of ADIPINT expression extracted from the FANTOM5 database. Samples with detected expression (counts per million (cpm)) are plotted, 1829 samples were investigated. g Expression of ADIPINT (n = 3) in fractionated hADSC. NEAT1 (n = 6) and GAPDH (n = 6) were used to validate the nuclear and cytoplasmic fractions, respectively. h RNA-FISH for ADIPINT in differentiated hADSC, staining for nuclei (HOECHST-Blue) and lipid (BODIPY-Green) alongside fluorescent probes targeting ADIPINT (Yellow). The experiment was performed three times with similar results. Scale bar, 10 µm. Data are presented as mean values +/- SD for n < 6 and SEM for n ≥ 6. *p < 0.05, **p < 0.01, ****p < 0.0001. Source data are provided as a Source Data file.