Fig. 5. Pyruvate carboxylase inhibition recapitulates the effects on lipid metabolism as seen with ADIPINT knockdown.

a (n = 3–4), d (n = 3–5) Glycerol release (measure of basal lipolysis) b (n = 3–4), e (n = 3–5) intracellular triglyceride (TG) content and c (n = 4), f (n = 4) insulin-stimulated [¹⁴C] acetate incorporation into intracellular lipid (measure of lipid synthesis) in differentiated hADSC incubated with varying concentrations of (a–c) oxalate or (d–f) avidin compared to vehicle treated cells. ADIPINT knockdown using GapmeR 1 was used as a positive control for the effects on glycerol release, triglyceride content and lipid synthesis in oxalate treated cells. g PC RNA (n = 3) and h protein expression following siPC knockdown compared with siNC transfected hADSC. i glycerol release (n = 5) j intracellular triglyceride content (n = 4) and k insulin-stimulated [¹⁴C] acetate incorporation into intracellular lipid after PC knockown (n = 3). l The intracellular triglyceride content of hADSC after + \− avidin treatment and knockdown of either PC or ADIPINT. siADIPINT and siPC cells were compared with siNC cells treated with 0.1 µM avidin (n = 4). a–g and i–l each data point represents an individual experiment. One-way ANOVA with Dunnett’s post-hoc test assessed significance. Data are presented as mean values + /- SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.