Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 27;185(13):2279–2291.e17. doi: 10.1016/j.cell.2022.05.019

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Elsevier Inc.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Figure S4 — Characterization of CCoV-HuPn-2018 domain 0-, A-, and B-I53-50 nanoparticles, related to Figure 3

Nanoparticles (NPs) were prepared by incubation of domain 0-I53-50A, domain A-I53-50A, or domain B-I53-50A trimers with pentameric I53-50B at molar ratios of 6 (1-μM domain 0-I53-50A + 5-μM I53-50A):1 1:1 and 1:1, respectively, for 30 min at room temperature. Formation of the domain-0-containing NPs required mixing domain 0-I53-50A and I53-50A, whereas only domain A-I53-50A or domain B-I53-50A were used (besides I53-50B) for assembly of domain-A- or domain-B-containing NPs, respectively.

(A–C) Representative electron micrographs of negatively stained CCoV-HuPn-2018 domain 0-, A-, or B-I53-50 NPs. Scale bars, 200 nm.

(D) SDS-PAGE analysis of purified nanoparticles.