FIG. 3.

Partial nucleotide sequence of the Z. mobilis DNA fragment in pLOI2183 which served as a surrogate promoter. The full sequence has been assigned GenBank accession number AF109242. Sequence numbering began with the base adjacent to the upstream Sau3A1 site (GATC). Two transcriptional start sites were identified by primer extension analysis and are marked with a “#” symbol. Putative −35 and −10 regions are marked by double and single underlines, respectively. Vector and celZ DNA are shown as lowercase letters. The Shine-Dalgarno region and the celZ translational start are marked in boldface.