TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Description	Source or reference
Strains
Z. mobilis CP4	Prototrophic	31
E. coli
DH5α	lacZ M15 recA	Bethesda Research Laboratory
B	Prototrophic	ATCC 11303
HB101	recA lacY recA	ATCC 37159
Plasmids
pUC19	bla cloning vector	New England Biolabs
pST76-K	kan low copy number, temperature sensitive	33
pRK2013	kan mobilizing helper plasmid (mob+)	ATCC
pCPP2006	Spr, ca. 40-kbp plasmid carrying the complete out genes from E. chrysanthemi EC16	15
pLOI1620	bla celZ	4
pLOI2164	pLOI1620 with BamHI site removed (Klenow)	This study
pLOI2170	NdeI-HindIII fragment (promoterless celZ) from pLOI2164 cloned into pUC19	This study
pLOI2171	BamHI-SphI fragment (promoterless celZ) from pLOI2170 cloned into pST76-K	This study
pLOI2173	EcoRI-SphI fragment (celZ with native promoter) from pLOI2164 cloned into pST76-K	This study
pLOI2174	EcoRI-BamHI fragment (gap promoter) cloned into pLOI2171	This study
pLOI2175	EcoRI-BamHI fragment (eno promoter) cloned into pLOI2171	This study
pLOI2177	Random Sau3A1 Z. mobilis DNA fragment cloned into pLOI2171	This study
pLOI2178	Random Sau3A1 Z. mobilis DNA fragment cloned into pLOI2171	This study
pLOI2179	Random Sau3A1 Z. mobilis DNA fragment cloned into pLOI2171	This study
pLOI2180	Random Sau3A1 Z. mobilis DNA fragment cloned into pLOI2171	This study
pLOI2181	Random Sau3A1 Z. mobilis DNA fragment cloned into pLOI2171	This study
pLOI2182	Random Sau3A1 Z. mobilis DNA fragment cloned into pLOI2171	This study
pLOI2183	Random Sau3A1 Z. mobilis DNA fragment cloned into pLOI2171	This study
pLOI2184	Random Sau3A1 Z. mobilis DNA fragment cloned into pLOI2171	This study
pLOI2196	pLOI2177 fused into pUC19 at the PstI site	This study
pLOI2197	pLOI2180 fused into pUC19 at the PstI site	This study
pLOI2198	pLOI2182 fused into pUC19 at the PstI site	This study
pLOI2199	pLOI2183 fused into pUC19 at the PstI site	This study
pLOI2307	EcoRI-SphI fragment from pLOI2183 cloned into pUC19	This study