TABLE 2.
Evaluation of promoter strength for celZ expression in E. coli by using CMC indicator plates
| Plasmids |
E. coli DH5α host
|
E. coli B host
|
||||
|---|---|---|---|---|---|---|
| No. of plasmidsa | CMC zone diam (mm)b | % Native promoter (100 · R2x/R2c)c | No. of plasmids | CMC zone diam (mm) | % Native promoter (100 · R2x/R2c) | |
| pLOI2171 (promoterless) | 1 | 0 | ||||
| pLOI2173 (native promoter) | 1 | 5.0 | 100 | 1 | 4.5 | 100 |
| pLOI2174 (gap promoter) | 1 | 4.0 | 77 | 1 | 3.5 | 60 |
| pLOI2175 (eno promoter) | 1 | 3.0 | 43 | 1 | 2.8 | 35 |
| Z. mobilis promoters | ||||||
| Group I | 5 | 13.0 | 676 | 4 | 10.8–11.3 | 570–625 |
| Group II | 14 | 9.0–11.0 | 324–484 | 17 | 9.0–10.5 | 445–545 |
| Group III | 56 | 6.0–9.0 | 144–324 | 54 | 5.0–8.8 | 125–375 |
a
The number of clones with the indicated range of activities.
b
The average size of the diameters from three CMC digestion zones.
c
R2x is the square of the radius of the clear zone with the test plasmid; R2c is the square of the radius of the clear zone for the control (pLOI2173).